Using RAPD markers for Detection the genetic diversity of E. coli isolates from Kirkuk hospitals

نویسندگان

چکیده

300 samples from different infection sourcel for a patients and revisers in Azadi hospital, Kirkuk generic children various ages both genders, to isolate Escherichia coli bacteria diagnosis it, study the genetic variety which distribution clinical by (140) urine samples, (45) diarrhea (40) wound swab, burn (35) otitis media infection.
 The growth isolation on cultural was diagnosed appearance, microscopical, characteristics. results showed that (75) about (38.65%) refers E. distributed (9) at ratio (31%) (6) (26.1) (4) (19%), (13) stool (40.6%), (43) (84.3%) urinary tract (UTI).
 sensitivity of ecological isolations studied nine antibiotics antibiotics, broadly. Where highest resistance (ampicillin cephalexin) reaching rate (97.3%) (92%), respectively. While lowest (Amikacin) (17.3%).
 It has been selected 24 isolations, depending here many antibiotics. To variation these markers it used type molecular is PCR technical is: randomly Amplified polymorphic DNA (RAPD).

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ژورنال

عنوان ژورنال: Ma?alla? Tikr?t li-l-?ul?m al-?irfa?

سال: 2023

ISSN: ['2415-1726', '1813-1662']

DOI: https://doi.org/10.25130/tjps.v23i5.589